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You Searched For: Protein Purification Reagents

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.

Protein purification reagents are for use in isolating one or more proteins from a complex mixture, usually cells, tissues, or whole organisms. This process is vital for function and structure characterization, as well as identifying interactions of the protein of interest. Important during the many steps, the protein purification reagents assist with cell lysis, separating the soluble protein components from cell debris, and finally separating the protein of interest from product- and process-related impurities.


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Product Image-8181-96

J.T.Baker® BAKERBOND®, Protein Precipitation Plates

Supplier: Avantor
Description: J.T.Baker® BAKERBOND® protein precipitation plates enable fast protein removal from biological samples.

Product Image-8182-02

J.T.Baker® BAKERBOND®, Supported Liquid Extraction Plates

Supplier: Avantor
Description: J.T.Baker® BAKERBOND® supported liquid extraction (SLE) plates are an ideal and faster alternative to traditional liquid-liquid extraction methods and allow excellent recoveries of analytes from biological matrices.

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Pep-Extract™

Catalog Number: (786-243)
Supplier: G-Biosciences
Supplier Article Number : 786-243
Description: Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.
UOM: 1 * 100 Tests
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Proteomic protein controls

Supplier: G-Biosciences
Description: Protein preparations specifically prepared for standardising electrophoresis methods and protocols. Substantially free from non-protein agents such as nucleic acids, detergents, salts, lipids and other common laboratory agents, the protein preparation has low conductivity (<50 μs). Supplied as dry protein pellets, simply re-hydrate and use. Proteomic protein sample controls are available for animal cells, <i>E.coli</i>, yeast, plant or as a control set that contains one 2 mg vial of each.

Product Image-786-391

Immobilised D-galactose for lectin purification

Catalog Number: (786-391)
Supplier: G-Biosciences
Supplier Article Number : 786-391
Description: Immobilised D-Galactose is designed for the rapid purification of lectins, galactosidases and other galactose-binding molecules. The resin is ideal for the purification of agglutinins, lectins, toxins, glactose-binding, N-acetylgalactosaminebinding or carbohydrate binding molecules. Specific applications include the purification of galactosidases, C‐type lectins, enterotoxins and cholera toxin.
UOM: 1 * 5 mL
Product Image-786-218

Concanavalin A agarose

Supplier: G-Biosciences
Description: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Certificates

Product Image-786-167

Jacalin for IgA purification

Supplier: G-Biosciences
Description: Jacalin, or <i>Artocarpus integrifolia</i> lectin, is a tetrameric two-chain lectin with a molecular weight of 66 kDa. It is a α-D-galactose binding lectin purified from jack fruit (<i>Artocarpus integrifolia</i>) seeds.

Product Image-PIER88328

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific
Description: The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Certificates

Product Image-PIER77720

TCEP solution, neutral pH, Bond-Breaker™ Pierce™

Catalog Number: (PIER77720)
Supplier: Thermo Fisher Scientific
Supplier Article Number : 77720
Description: Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.
UOM: 1 * 5 mL

MSDS Certificates


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Recombinant protein L, peroxidase conjugated, Pierce™

Catalog Number: (PIER32420)
Supplier: Thermo Fisher Scientific
Supplier Article Number : 32420
Description: Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
UOM: 1 * 0,5 mg
Image Unavailable-PIER32400

Recombinant protein A, peroxidase conjugated, Pierce™

Catalog Number: (PIER32400)
Supplier: Thermo Fisher Scientific
Supplier Article Number : 32400
Description: Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.
UOM: 1 * 1 mg

MSDS


Product Image-PIER21184

Recombinant protein A, Pierce™

Supplier: Thermo Fisher Scientific
Description: Pierce™ Recombinant Protein A is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols.

Product Image-PIER22711

Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Supplier: Thermo Fisher Scientific
Description: Pierce™ MM(PEG)<sub>n</sub> reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

Product Image-PIER78115

Inclusion body solubilisation reagent, Pierce™

Catalog Number: (PIER78115)
Supplier: Thermo Fisher Scientific
Supplier Article Number : 78115
Description: Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.
UOM: 1 * 100 mL
Product Image-PIER44881

Immobilised ficin (agarose resin), Pierce™

Catalog Number: (PIER44881)
Supplier: Thermo Fisher Scientific
Supplier Article Number : 44881
Description: Pierce™ Immobilised Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilised onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.
UOM: 1 * 5 mL
Product Image-PIER21181

Pierce™ Protein A

Supplier: Thermo Fisher Scientific
Description: Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory.affairs.ch@vwr.com
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