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Ditiotreitolo (reattivo di Cleland) ≥99.4% per biotecnologie

Fornitore: VWR Chemicals
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Synonyms: DTT

0281-25GEA 218 CHF
0281-100G 0281-25G 0281-1KG 0281-5G 0281-2500
Ditiotreitolo (reattivo di Cleland) ≥99.4% per biotecnologie
Ditiotreitolo (reattivo di Cleland)

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.


DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.


It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Formula: C₄H₁₀O₂S₂
Peso molecolare: 154,25 g/mol
Punto di ebollizione: < 125 °C (1013 hPa)
Punto di fusione: 42…44 °C
Flash Pt: >110 °C (coppa aperta)
Temperatura di stoccaggio: Refrigerata
MDL Number: MFCD00004877
CAS Number: 3483-12-3
EINECS: 222-468-7
https://ch.vwr.com/stibo/web/std.lang.all/98/19/2299819.jpg

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Risultati del test di specificazione

Abs.@283nm (0.02M, Acqua) ≤0.05
DNasi Assente
Perdita per essiccamento <0.5%
Punto di fusione 39 - 43 °C
Ossidasi DTT <0.5%
Proteasi Assente
Purezza (-SH Contenuto) >99.4%
RNasi Assente
Solubilità (5%, Acqua) Conforme

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