Ditiotreitolo (reattivo di Cleland) ≥99.4% per biotecnologie
Fornitore: VWR Chemicals
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Attenzione
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Synonyms:
DTT
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.
DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.
It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Formula:
C₄H₁₀O₂S₂ Peso molecolare: 154,25 g/mol Punto di ebollizione: < 125 °C (1013 hPa) Punto di fusione: 42…44 °C Flash Pt: >110 °C (coppa aperta) Temperatura di stoccaggio: Refrigerata |
MDL Number:
MFCD00004877 CAS Number: 3483-12-3 EINECS: 222-468-7 |
Risultati del test di specificazione
Abs.@283nm (0.02M, Acqua) | ≤0.05 |
DNasi | Assente |
Perdita per essiccamento | <0.5% |
Punto di fusione | 39 - 43 °C |
Ossidasi DTT | <0.5% |
Proteasi | Assente |
Purezza (-SH Contenuto) | >99.4% |
RNasi | Assente |
Solubilità (5%, Acqua) | Conforme |
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