Human mesenchymal stem cells from bone marrow

Lieferant: Charles River Laboratories Cell Solutions
BM012C-1EA 1540 CHF
BM012C-1
Human mesenchymal stem cells from bone marrow
Zellen
Mesenchymal stem cells (MSC) are cultured from freshly collected adult human bone marrow. MSCs are isolated by adherence from bone marrow mononuclear cells, isolated using a density gradient. Adherent cells were cultured in MEM-alpha, supplemented with L-glutamine and Human Platelet Lysate. Bone marrow aspirate is collected from healthy human volunteer donors under IRB approved informed consent and drawn from the posterior iliac crest using a syringe containing anticoagulant.

  • Purity >90% by flow cytometry
  • Test negative for Hepatitis B and C, HIV 1/2, HTLV- I/II, HCV/HBV, WNV, Trypanasoma cruzi, and Syphilis
  • Cryopreserved in CryoStor® CS10 media

Mesenchymal stem cells are multipotent stromal cells that can differentiate into a variety of cell types including osteoblasts (bone), chondrocytes (cartilage), adipocytes (fat), myocytes (muscle), and neurons (nerve). Because mesenchymal stem cells are undifferentiated cells, they are able to self-renew and have a high proliferative capacity. Mesenchymal stem cells can be isolated from a variety of different tissues, such as bone marrow, adipose tissue, dental pulp, placenta, and umbilical cord blood.

Storage: Either prepare cells for long term storage in liquid nitrogen vapour phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short term storage of cells (less than 2 weeks) at –80 °C is acceptable, but should be minimised to ensure maximum stability. Once thawed, samples must be used immediately.

Bitte beachten Intended for research use only and not for direct therapeutic or diagnostic use in human or animals, or for further manufacturing of therapeutic products or devices.
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