The Biosensis NGFR/p75ECD Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of mouse p75 neurotrophin receptor in less than 4 hours in cell culture supernatants and urine only if used as directed.
This NGFR/p75ECD ELISA kit employs a recombinant mouse p75ECD-Fc chimera as standard. While there is a current lack of a true mouse p75ECD standard, this ELISA kit allows quantification of mouse p75ECD as p75ECD–Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75ECD.
The purpose of this kit is the in vitro quantitative determination of mouse p75ECD in samples such as cell culture supernatants and urine. This kit has not been tested for other applications.
This kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a goat anti-p75ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards.
Please refer to the kit protocol for specific use instructions for each substrate application.
Lieferumfang: Each kit contains 1 or 2 × 96 pre-coated strip plates, protein standards, detection reagents, wash and sample buffers and detailed instructions.
Bitte beachten For research use only. Not for diagnostic or clinical purposes.
