DNA extraction, illustra™ Nucleon™ PhytoPure™Supplier: Cytiva
RPN8510EA 322 CHFRPN8510 RPN8511
DNA extraction, illustra™ Nucleon™ PhytoPure™
Nucleic Acid Reagents Nucleic Acid Purification Kits and Reagents
Nucleon™ PhytoPure™ enables rapid chloroform extraction of high-quality, high molecular weight genomic DNA from plant and fungal samples with efficient removal of protein as well as polysaccharides.
- PhytoPure™ resin binds plant polysaccharides ensuring high yields of high quality plant genomic DNA in only 60 minutes
- Successfully extracts DNA from many plant species, starting with fresh, frozen, or freeze dried material
- Two kit sizes provide economical scale-up using the same phenol-free protocol
Polysaccharides are very common contaminants in plant DNA extracts, and often result in difficult-to-handle, "slimy" DNA pellets.
illustra™ Nucleon™ PhytoPure™ extracts genomic DNA from plant and fungal samples in a fraction of the time taken by conventional methods, while efficiently removing proteins and polysaccharides. The Nucleon™ PhytoPure™ proprietary resin is added following cell lysis with potassium SDS, and a single chloroform extraction. Aspiration of the top layer of nucleic acids is simplified and volume of usable sample maximised by a barrier formed between the two layers. The combination of purity and high molecular weight makes the recovered DNA suitable for a variety of molecular biology applications, including whole genome amplification.
Sample types include fresh, frozen or freeze dried samples of Arabidopsis thaliana, Brassica oleracea, Brassica napus, Capsicum anuum, Capsicum frutescens, cereals (barley, maize, rye, wheat), Cocos nucifera, Helianthus annus, Helianthus tuberosus, Hevea braziliensis, Irvingia gabonensis and Lotus japonicus.
Although most plant DNA extraction techniques are effective at removing proteins, they are much less successful with polysaccharides.