Chymotrypsin™, sequencing grade

Supplier: G-Biosciences
786-13EA 593 CHF
Chymotrypsin™, sequencing grade
Nucleic Acid Reagents Sanger Sequencing Reagents
SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.

  • Modified chymotrypsin for sequence analysis
  • Resistant to autolysis and degradation
  • High specificity, free from other known endopeptidases
  • Consistency of activity levels from lot to lot for reproducible

SG-Chymotrypsin™ is first treated with TLCK to inhibit trypsin that may be present and then subjected to an extensive purification process to remove contaminating protease and chymotryptic autolysis by-products. The highly purified enzyme is then chemically modified to increase its resistance to autolysis and stability.

For protein digestion SG-Chymotrypsin™ is added to the protein at a ratio of 1:200 to 1:50, by weight, in a standard digestion buffer. Incubate at 25 - 30 °C for 1 to 10 hours, but can be extended to 24 hours, due to the extended life of the SG-Chymotrypsin™. We recommend choosing a ratio of enzyme to protein that allows for the shortest incubation time possible. This will reduce or eliminate the catalysed hydrolysis of peptide bonds with non-aromatic amino acid residues.
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