Guanidinium thiocyanate for molecular biologySupplier: Thermo Scientific
, Guanidinium rhodanide
Guanidine thiocyanate is used for RNA isolation and can solubilise proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.
Soluble in H₂O (1420 g/L) at 20°C, and ethanol (5%).
Caution: Light Sensitive. Keep container tightly closed and store away from oxidising agents.
MW: 118.16 g/mol
Melting Pt: 118…122 °C
Storage Temperature: Ambient
CAS Number: 593-84-0
Specification Test Results
|Appearance (Colour)||White to off-white|
|Assay (Titration)||99.0% min.|
|Protease activity||None detected|
|UV/Vis A280 (70%, water)||0.8|
|UV/Vis A300 (6M, water)||0.1|
|UV/Vis A410 (6M, water)||0.1|