PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.
PerfeCta® PreAmp SuperMix delivers unbiased pre-amplification of up to 100 target sequences from as little as 100 pg of total RNA. It is compatible with both TaqMan® 5’-nuclease probes as well as ds-DNA binding dye (i.e. SYBR® Green I) qPCR detection chemistries.
A key component of PerfeCTa® PreAmp SuperMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is resistant to PCR inhibitors and provides an extremely stringent automatic hot start allowing reaction assembly, and temporary storage, at room temperature prior to pre-amplification.
Delivery information: Each kit contains 5X reaction buffer containing optimised concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), hot start DNA polymerase, AccuVue™ blue tracer dye, and stabilisers.